<var id="zf1xp"></var>
<menuitem id="zf1xp"></menuitem>
<var id="zf1xp"></var>
<menuitem id="zf1xp"></menuitem>
<var id="zf1xp"><strike id="zf1xp"></strike></var>
<cite id="zf1xp"><strike id="zf1xp"></strike></cite>
<menuitem id="zf1xp"><dl id="zf1xp"><progress id="zf1xp"></progress></dl></menuitem><var id="zf1xp"></var>
<var id="zf1xp"><strike id="zf1xp"><listing id="zf1xp"></listing></strike></var>
<var id="zf1xp"><dl id="zf1xp"></dl></var>
<var id="zf1xp"></var>
<cite id="zf1xp"><strike id="zf1xp"></strike></cite><var id="zf1xp"></var>
<var id="zf1xp"><video id="zf1xp"></video></var><var id="zf1xp"><strike id="zf1xp"><listing id="zf1xp"></listing></strike></var>
<var id="zf1xp"></var>
<menuitem id="zf1xp"></menuitem>
<var id="zf1xp"></var>
<cite id="zf1xp"><strike id="zf1xp"></strike></cite>
<var id="zf1xp"></var>
<var id="zf1xp"><strike id="zf1xp"><listing id="zf1xp"></listing></strike></var>

[1]方海仙,梅文泉,杨东顺,等.紫外分光光度法快速测定山葵中异硫氰酸酯的研究[J].中国调味品,2016,(10):109-111.[doi:10.3969/j.issn.1000-9973.2016.10.024]
 FANG Hai-xian,MEI Wen-quan,et al.Study on Rapid Determination of Isothiocyanates in Wasabia japonica Mastum by Ultraviolet Spectrometry[J].CHINA CONDIMENT,2016,(10):109-111.[doi:10.3969/j.issn.1000-9973.2016.10.024]
点击复制

紫外分光光度法快速测定山葵中异硫氰酸酯的研究
分享到:

《中国调味品》[ISSN:1000-9973/CN:23-1299/TS]

卷:
期数:
2016年10期
页码:
109-111
栏目:
分析检测
出版日期:
1900-01-01

文章信息/Info

Title:
Study on Rapid Determination of Isothiocyanates in Wasabia japonica Mastum by Ultraviolet Spectrometry
文章编号:
1000-9973(2016)10-0109-03
作者:
方海仙12梅文泉12杨东顺12汪禄祥12*
1.云南省农业科学院质量标准与检测技术研究所,昆明 650223;
2.农业部农产品质量监督检验测试中心,昆明 650223
Author(s):
FANG Hai-xian1 2 MEI Wen-quan1 2 YANG Dong-shun1 2 WANG Lu-xiang1 2*
1.Agri-Food Quality Standard and Testing Technology Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223, China;
2.Supervision & Testing Center for Farm Products Quality, Ministry of Agriculture, Kunming 650223, China
关键词:
山葵 异硫氰酸酯 紫外分光光度法 快速测定
Keywords:
Wasabi japonica Mastum isothiocyanates ultraviolet spectrometry rapid determination
分类号:
TS201.2
DOI:
10.3969/j.issn.1000-9973.2016.10.024
摘要:
目的:建立一种快速测定山葵中异硫氰酸酯含量的方法。方法:采用紫外分光光度法,以烯丙基异硫氰酸酯为对照品,测定249 nm处的吸光度。结果:对照品在0~80 μg/mL范围内,吸光度呈良好的线性关系(R=0.9997),回归方程为Y=0.0127X-0.013,加标回收率在86.7%~98.8%之间,方法相对标准偏差为1.09%~3.33%,测得山葵中异硫氰酸酯含量为0.079%。结论:该方法简单快速、准确度高、重复性好,为进一步开发利用山葵提供了理论基础。
Abstract:
Objective: A method for the rapid quantification of isothiocyanates in Wasabi japonica Mastum is established. Methods: In this test, the content of isothiocyanates is detected by ultraviolet spectrometry, allyl isothiocyanate is regarded as the standard, and the maximum absorption wavelength is at 249 nm. Results: The good linearity between concentration and absorbance of the standard is within the range of 0~80 μg/mL with a relativity coefficient of 0.9997, the linear regression equation is: Y=0.0127 X-0.013. The recovery rate is 86.7%~98.8% and the relative standard deviation is 1.09%~3.33%, the isothiocyanates content in Wasabi japonica Mastum is 0.079%. Conclusions: The method is simple and fast, has high accuracy and good repeatability, and it has provided theoretical basis for the further development and utilization of Wasabi japonica Mastum.

参考文献/References:

[1]Depree J A, Howard T M,Savage G P.Flavour andpharmaceuticalproperties of the volatile sulphur compounds of wasabi(Wasabia japonica)[J].Food Research International, 1998, 31(5):329-337.
[2]Ravindran P N, Pillai G S, Divakaran M.28-Other herbs and spices: mango ginger to wasabi[M].Handbook of Herbs and Spices(Second Edition), 2012: 557-582.
[3]郑家文,杨祚斌.山葵栽培技术[J].西南农业学报,2004,17:111-115.
[4]陆礼和,陈颢,孙皓,等.山葵营养成分分析[J].云南化工,2011,38(4):17-18.
[5]殷帅文,何旭梅,曾建军,等.山葵保健酒化学成分气相色谱-质谱联用分析研究[J].时珍国医国药,2014,25(10):2333-2334.
[6]杜喜玲,蒲彪.山葵的风味物质及其功能应用研究进展[J].食品研究与开发,2008,29(1): 173-177.
[7]Il Shik Shin, Hideki Masuda, Kinae Naohide. Bactericidal activity of wasabi(Wasabia japonica)against Helicobacter pylori[J].International Journal of Food Microbiology, 2004, 94(3):255-261.
[8]姜子涛,张清峰,李荣.异硫氰酸酯的产生、化学性质及测定方法[J].中国调味品,2005(4):9-14.
[9]Yoko Fuke, Shoko Shinoda, Ikuko Nagata, et al. Preventive effect of oral administration of 6-(methylsulfinyl)hexyl isothiocyanate derived from wasabi(Wasabia japonica Matsum)against pulmonary metastasis of B16-BL6 mouse melanoma cells[J].Cancer Detection and Prevention, 2006, 30(2):174-179.
[10]毕明芳.山葵风味成分的提取及活性研究[D].广州:广东工业大学,2015.
[11]胡国伟,杨瑛洁,李淑燕,等.异硫氰酸酯分析方法研究进展[J].食品工业科技,2011, 32(12):524-527.
[12]Tamanna Sultana, David L McNeil, Noel G Porter, et al. Investigation of isothiocyanate yield from flowering and non-flowering tissues of wasabi grown in a flooded system[J].Journal of Food Composition and Analysis, 2003,16(6):637-646.
[13]张清峰,姜子涛,张久春,等.紫外分光光度法测定辣根及芥末制品中异硫氰酸酯含量的研究[J].中国调味品,2005(6):15-20.

备注/Memo

备注/Memo:
作者简介:方海仙(1988-),女,云南师宗人,助理研究员,硕士,主要从事食品安全检测工作。
更新日期/Last Update: 1900-01-01
一号彩票